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enterokinase  (New England Biolabs)


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    Structured Review

    New England Biolabs enterokinase
    Enterokinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/enterokinase+light+chain/Enterokinase%2C+light+chain/us12599649-2015-16-17
    Average 96 stars, based on 287 article reviews
    enterokinase - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Production of active human iduronate-2-sulfatase (IDS) enzyme in Nicotiana benthamiana
    Article Snippet: .. 0, 50 mM NaCl, and 2 mM CaCl 2 ) and then incubated with 10 μL (50 U) enterokinase light chain (Cat. No. P8070, New England Biolabs, USA) at 25 o C for 16 h under gentle rotation at 150 rpm according to the manufacturer’s instructions. ..

    Article Title: Site‐Specific Quadruple‐Functionalised Antibodies
    Article Snippet: .. The resulting protein solution was analysed by LC-MS. S6.2 FLAG cleavage Antibody solution (2 mg/mL in Tris (50 mM, pH 7.5), NaCl (150 mM), CaCl2 (10 mM) buffer) was incubated overnight with enterokinase light chain (2.5 μL of 16,000 units/mL per 2 mg of protein, NEB) at 25 °C. .. The resulting protein solution was analysed by LC-MS. S6.3 GlySERIAS cleavage Antibody solution (20 μL, 1 mg/mL in TBS pH 7.6) was incubated with GlySERIAS (40 units, Genovis) at 37 °C for 1 hour.

    Article Title: Thiocholine-Mediated One-Pot Peptide Ligation and Desulfurization.
    Article Snippet: Briefly, His6-tagged H3.1 mutant protein was isolated and purified under denaturing conditions using nickel–nitrilotriacetic acid agarose (Ni–NTA) resin (QIAGEN, Hilden, Germany). .. The enterokinase light chain (New England BioLabs, Ipswich, MA, USA) was added to the purified H3.1 mutant protein (0.57 ng/mg of H3.1 mutant) to remove the His6-tagged N-terminal region (aa 1–28), and the mixture was incubated at 20 ◦C for 16 h. The reaction product was then mixed with 1.5 times the volume of cation exchange buffer (20 mM NaOAc pH5.2, 6 M urea, 10 mM 2-mercaptoethanol, 1 mM EDTA) and subjected to a HiPrep SP HP 16/10 cation exchange column (Cytiva, Muskegon, MI, USA). ..

    Article Title: Production of active human iduronate-2-sulfatase (IDS) enzyme in Nicotiana benthamiana.
    Article Snippet: .. 0, 50 mM NaCl, and 2 mM CaCl2) and then incubated with 10 μL (50 U) enterokinase light chain (Cat. No. P8070, New England Biolabs, USA) at 25oC for 16 h under gentle rotation at 150 rpm according to the manufacturer’s instructions. ..

    Gentle:

    Article Title: Production of active human iduronate-2-sulfatase (IDS) enzyme in Nicotiana benthamiana
    Article Snippet: .. 0, 50 mM NaCl, and 2 mM CaCl 2 ) and then incubated with 10 μL (50 U) enterokinase light chain (Cat. No. P8070, New England Biolabs, USA) at 25 o C for 16 h under gentle rotation at 150 rpm according to the manufacturer’s instructions. ..

    Article Title: Production of active human iduronate-2-sulfatase (IDS) enzyme in Nicotiana benthamiana.
    Article Snippet: .. 0, 50 mM NaCl, and 2 mM CaCl2) and then incubated with 10 μL (50 U) enterokinase light chain (Cat. No. P8070, New England Biolabs, USA) at 25oC for 16 h under gentle rotation at 150 rpm according to the manufacturer’s instructions. ..

    In Vitro:

    Article Title: Supporting Information: Multiplexed enzyme activity-based probe display via hybridization
    Article Snippet: .. Hexafluoroisopropanol (HFIP), trifluoroacetic acid (TFA), triisopropylsilane (TIPS), α- cyano-4-hydroxycinnamic acid (HCCA), 2’,4’,6’ trihydroxyacetophenone monohydrate (THAP), Dynabeads M-270 carboxylic acid (Life Technologies, Carlsbad, CA), Dynabeads M-270 Streptavidin (Life Technologies), Dynabeads M-270 amine (Life Technologies), triethylamine acetate (TEAA, 2 M, Life Technologies), N,N’-diisopropylcarbodiimide (DIC, Acrōs Organics, Fair Lawn, NJ), L(+)-ascorbic acid (Acrōs Organics), 1-[(1-(Cyano- 2-ethoxy-2oxoethylideneaminooxy) dimethylaminomorpholino)] uronium hexafluorophosphate (COMU, Acros Organics, Geel, Belgium), Fmoc-Ala-OH (AnaSpec, Fremont, CA), Fmoc-Arg(Pbf)OH (AnaSpec), Fmoc-(PEG)2-OH (Anaspec), Fmoc-Gly-OH (AnaSpec), cbzIPR-R110 fluorogenic probe (Anaspec), Fmoc-citrulline-OH (Anaspec), Fmoc-Cys(Dpm)-OH (Bachem, Torrance, CA), Fmoc-Ile-OH (CEM, Matthews, NC), Fmoc-Cys(Trt)-OH (CEM), Fmoc- S-1 Tyr(tBu)-OH (CEM), Fmoc-Pro-OH (CEM), Fmoc-propargylglycine (Bachem, Torrance, CA), Fmoc-Trp(Boc)-OH (CEM), dimethylformamide (DMF, Thermo Fisher Scientific, Waltham, MA), dichloromethane (DCM, Thermo Fisher Scientific), N,N-diisopropylethylamine (DIEA, Thermo Fisher Scientific), tris(2-carboxyethyl)phosphine hydrochloride (TCEP HCl, Thermo Fisher Scientific), cyanine 5 N-hydroxysuccinimidyl ester (Cy5-NHS, Lumiprobe, Hallandale Beach, FL), 2’-deoxyribonucleotide triphosphate (dNTP, set of dATP, dTTP, dGTP, dCTP, Promega Corp., Milwaukee, WI), 5-(and-6)-carboxyrhodamine 110 (Exciton, Inc., Dayton, OH), KF-6038 (Shin-Etsu, Tokyo, Japan), KF-6012 (Shin-Etsu), DMF-A-6cs (Shin-Etsu), Taq DNA polymerase (New England Biolabs, Ipswich, MA), T4 DNA ligase (New England Biolabs), NcoI (New England Biolabs), BlpI (New England Biolabs), EcoRI (New England Biolabs), BamHI (New England Biolabs), PURExpress In Vitro Protein Synthesis kit (New England Biolabs), PURExpress Disulfide Bond Enhancer (New England Biolabs), and enterokinase light chain (EK, New England Biolabs) were used as provided. ..

    other:

    Article Title: Peptide Barcodes for miRNA activity assessment in mammalian cells
    Article Snippet: For anti-FLAG immunoaffinity purification, the DYKDDDDK Fab-Trap Agarose Kit (ChromoTek, Cat# ffak-20) was used.

    Purification:

    Article Title: Thiocholine-Mediated One-Pot Peptide Ligation and Desulfurization.
    Article Snippet: Briefly, His6-tagged H3.1 mutant protein was isolated and purified under denaturing conditions using nickel–nitrilotriacetic acid agarose (Ni–NTA) resin (QIAGEN, Hilden, Germany). .. The enterokinase light chain (New England BioLabs, Ipswich, MA, USA) was added to the purified H3.1 mutant protein (0.57 ng/mg of H3.1 mutant) to remove the His6-tagged N-terminal region (aa 1–28), and the mixture was incubated at 20 ◦C for 16 h. The reaction product was then mixed with 1.5 times the volume of cation exchange buffer (20 mM NaOAc pH5.2, 6 M urea, 10 mM 2-mercaptoethanol, 1 mM EDTA) and subjected to a HiPrep SP HP 16/10 cation exchange column (Cytiva, Muskegon, MI, USA). ..

    Mutagenesis:

    Article Title: Thiocholine-Mediated One-Pot Peptide Ligation and Desulfurization.
    Article Snippet: Briefly, His6-tagged H3.1 mutant protein was isolated and purified under denaturing conditions using nickel–nitrilotriacetic acid agarose (Ni–NTA) resin (QIAGEN, Hilden, Germany). .. The enterokinase light chain (New England BioLabs, Ipswich, MA, USA) was added to the purified H3.1 mutant protein (0.57 ng/mg of H3.1 mutant) to remove the His6-tagged N-terminal region (aa 1–28), and the mixture was incubated at 20 ◦C for 16 h. The reaction product was then mixed with 1.5 times the volume of cation exchange buffer (20 mM NaOAc pH5.2, 6 M urea, 10 mM 2-mercaptoethanol, 1 mM EDTA) and subjected to a HiPrep SP HP 16/10 cation exchange column (Cytiva, Muskegon, MI, USA). ..



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